Isolation and Purification of Adult Mouse Cardiomyocytes by Langendorff Perfusion and Gravity Sedimentation

cardiomyocytes per heart with a high proportion of rod-shaped cells (91.2% ± 2.11%), and a high purity (the percentage of cTNT⁺DAPI⁺ cells relative to DAPI⁺ cells was approximately 97.47% ± 1.365%), which exhibited clear sarcomeric structures. By providing a standardized and reproducible method for obtaining high-quality adult cardiomyocytes, this protocol effectively bridges the gap between in vitro studies and pathophysiological relevance, thereby supporting advanced cardiovascular research.