Nanocomposite-Based Dual Electrochemical Immunosensor for Simultaneous Detection of Intestinal Barrier Biomarkers: Intestinal Fatty Acid Binding Protein and Fecal Calprotectin

(FC) with a total assay time of 1 h 20 min and sample volume of just 5 μL, outperforming conventional ELISA in speed and efficiency. High repeatability, reproducibility, and accurate recovery in enriched fecal samples confirmed analytical robustness. By integrating multiplexed detection, nanostructured signal amplification, and robust electrode engineering, this immunosensor provides a rapid, sensitive, and low-volume platform for point-of-care and decentralized monitoring of IBD, enabling timely clinical decision-making and longitudinal patient management.