Decoding Sickle Cell Disorders Using Flow Injection Analysis Mass Spectrometry

RATIONALE: since these variants electrophoretically migrate close to each other. These cases often require hematological correlation or molecular confirmation. As molecular testing is not available in all laboratories, mass spectrometry (MS) may serve as an alternate approach for examining complex compound heterozygous sickle cell disorders. METHODS: ). Statistical analysis was performed using SPSS v27. RESULTS: /β, significantly discriminated against Group 1 from Group 3 (p < 0.001). While γ/β ratio significantly differentiated Group 1 from Group 2 (p < 0.001), the δ/α (%) ratio was superior for distinguishing Group 2 from Group 3 (p < 0.001). CONCLUSIONS: /β. Combined with capillary electrophoresis (CE), this approach enhances diagnostic resolution and may reduce the need for molecular testing.