Effects of Controlled Atmosphere Conditions on the Quality Characteristics, Physicochemical and Antioxidant Properties of Pork Bone Broth

Controlled atmosphere (CA) is widely employed to preserve perishable foods, yet its potential effects on the quality of thermally processed bone broth remain poorly understood. This work systematically investigated the influences of ventilation time (0, 1, and 3 s), ventilation frequency (30, 60, 90, and 110 cycles), and cooking duration (25, 30, 38, and 45 min) on the overall quality of pork bone broth. A single-factor experimental design was adopted with three replications per treatment. Results showed that CA treatment effectively improved the sensory properties of pork bone broth, including color, aroma, and taste. Different CA processing parameters differentially affected the accumulation of diglycerides, proteins, peptides, amino acids and lipid oxidation-related flavor compounds, as well as antioxidant activities and emulsion stability. Specifically, prolonged ventilation promoted the accumulation of diglycerides and medium-sized peptides (1-7 kDa) but concurrently reduced solids, fat content, and ABTS radical scavenging activity, suggesting a trade-off between flavor precursor generation and oxidative stability. Furthermore, most quality indicators initially increased with rising ventilation frequency but subsequently declined at excessive levels, with optimal values attained at moderate frequencies. Notably, CA conditions that enhanced the formation of desirable flavor compounds also increased the accumulation of lipid oxidation byproducts, highlighting a critical balance required for achieving optimal product quality. Ultimately, it was found that a ventilation time of 1 s, a ventilation frequency of 60 cycles per minute, and a cooking duration of 30 min maximized the benefits of controlled atmosphere (CA) processing, thereby achieving optimal sensory properties, flavor profiles and nutritional composition in pork bone broth. This study provides fundamental data to support the development and quality regulation of thermally processed meat broths.