Phage amplification-coupled CRISPR/Cas12a system for selective detection of viable E. coli in fresh produce
detection in the presence of a mixture of non-targeted bacteria. This work highlights the potential of phage-assisted CRISPR/Cas12a systems for rapid, low-cost, and reliable detection of viable foodborne pathogens in complex food matrices.
Authors
- Ahmed Y. El-Moghazy (ORCID: https://orcid.org/0000-0002-5743-7305)
- Nitin Nitin (ORCID: https://orcid.org/0000-0003-4839-4566)
- Nicharee Wisuthiphaet
Institutions
- University of California, Riverside (US)
- King Mongkut's University of Technology North Bangkok (TH)
- University of California, Davis (US)
Publication Details
- Journal
- Frontiers in Microbiology
- Published
- 2026-04-01
- DOI
- https://doi.org/10.3389/fmicb.2026.1770383
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Agriculture
- National Research Council of Thailand
- National Institute of Food and Agriculture