<i>Bacillus subtilis</i> Spore-Displayed Aminopeptidase from <i>Pseudomonas aeruginosa</i> Efficiently Degrades Pyrethroid Insecticides

aminopeptidase (PaAps) on the spore coat and evaluated its potential for pyrethroid degradation. Surface-anchored PaAps efficiently degraded various pyrethroids, with β-cypermethrin showing the highest removal. The enzyme exhibited remarkable thermal stability and pH tolerance, with optimal activity at 60 °C and pH 8.0, along with enhanced long-term storage stability. Soil microcosm studies revealed that PaAps not only accelerated β-cypermethrin degradation but also influenced the indigenous microbial community to enhance bioremediation. This study demonstrates a robust and environmentally sustainable biocatalytic strategy for pyrethroid detoxification, with promising applications in environmental remediation and food safety.