Electrochemical sensor based on MWCNTs/ZnONPs for detection of prostate specific antigen

² = 0.979). The proposed platform demonstrated good sensitivity, specificity, and operational stability, whereas negligible interference was observed for potential coexisting species, such as glucose, bovine serum albumin (BSA), and ascorbic acid. Glucose showed minimal current variation because of its weak electroactivity under the selected conditions, while BSA produced only a slight signal increase, likely due to limited protein adsorption onto the electrode surface. In contrast, ascorbic acid generated a relatively higher current response owing to its intrinsic electrochemical activity; however, its electrochemical profile remained distinguishable from the PSA signal. The good anti-interference performance was attributed to the combined effect of the conductive MWCNTs/ZnONPs platform and the BSA blocking layer, which reduced non-specific adsorption while preserving efficient electron transfer. Because of its easy fabrication, cost-effectiveness, and reliable analytical performance, the MWCNT/ZnONP/SPCE immunosensor is promising as a portable point-of-care device for prostate cancer screening.