Quantifying Protein Homodimer Affinities and the Effect of Molecular Glues and Interface Residues Using Native Mass Spectrometry

s in the high micromolar range, which allowed us to monitor the gradual decrease in homodimer affinity via mutation of crucial dimer interface contacts. Overall, qLILBID-MS is a sensitive, robust, fast, scalable, and cost-effective alternative to quantify protein/protein interactions, that can accelerate contemporary drug discovery workflows, e.g. the efficient screening for proximity inducing molecules like proteolysis targeting chimera (PROTACs) and molecular glues.

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Publication Details

Journal
Journal of the American Chemical Society
Published
2026-04-01
DOI
https://doi.org/10.1021/jacs.5c18602
Primary Topic
Protein Degradation and Inhibitors
Type
article
Field-Weighted Citation Impact
0.00

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article

Quantifying Protein Homodimer Affinities and the Effect of Molecular Glues and Interface Residues Using Native Mass Spectrometry

Nina Morgner, Ute A. Hellmich, Jonathan Schulte, Eric Schwegler
Journal of the American Chemical Society
Protein Degradation and Inhibitors
article

Quantifying Protein Homodimer Affinities and the Effect of Molecular Glues and Interface Residues Using Native Mass Spectrometry

Nina Morgner, Ute A. Hellmich, Jonathan Schulte, Eric Schwegler
article en

Abstract

s in the high micromolar range, which allowed us to monitor the gradual decrease in homodimer affinity via mutation of crucial dimer interface contacts. Overall, qLILBID-MS is a sensitive, robust, fast, scalable, and cost-effective alternative to quantify protein/protein interactions, that can accelerate contemporary drug discovery workflows, e.g. the efficient screening for proximity inducing molecules like proteolysis targeting chimera (PROTACs) and molecular glues.

Journal of the American Chemical SocietyVol. 148(14)
Goethe University Frankfurt (DE), Goethe-Institute United Kingdom (GB), Schiller International University (FR), Institute of Macromolecular Chemistry (UA), FuelCell Energy (United States) (US), Resonance Research (United States) (US)
Deutsche Forschungsgemeinschaft
Openalex Percentile: Top 33%
Protein Degradation and Inhibitors
DOIOpen Access PDFOpenAlex
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Quantifying Protein Homodimer Affinities and the Effect of Molecular Glues and Interface Residues Using Native Mass Spectrometry — Nina Morgner, Ute A. Hellmich, et al. · Journal of the American Chemical Society (2026) | TGRS Research Map | TGRS