First Establishment of LC–MS/MS Method for Quantitative Analysis of Pharmacokinetics and Tissue Distribution of Trilobatin—Comparison of Three Administration Modes

was also approximately 1 h after intraperitoneal administration. Compared to intravenous injection, the relative bioavailability of oral administration and intraperitoneal injection is only 0.004% and 0.3%, respectively. Tissue distribution results from the three administration routes indicated that trilobatin exhibits widespread tissue distribution. These findings provide a theoretical basis for further research on trilobatin. This study provides detailed insights into the pharmacokinetic and tissue distribution characteristics of trilobatin in rats for the first time, laying the foundation for further research on trilobatin as a potential new drug candidate.