Multi-omics identifies a T2DM-associated immune-regulatory network modulated by electroacupuncture

Background: Recent studies suggest a link between immune-related processes and type 2 diabetes (T2DM), yet the specific genes and mechanisms remain unclear. This study aimed to explore the mechanistic relationship by integrating multi-omics data. Methods: We identified T2DM-associated candidate genes through summary data-based Mendelian Randomization (SMR) and colocalization analyses using immune-related QTL data and large-scale GWAS data. We then functionally validated key genes in a high-fat diet-induced T2DM mouse model, assessing gene expression and the therapeutic effects of electroacupuncture (EA) using RT-qPCR. Results: , confirming their functional relevance. Conclusions: -in T2DM pathogenesis. The modulation of these genes by EA treatment provides direct evidence for the molecular mechanisms underlying its therapeutic effect on T2DM.