Determinants of metal import and specificity in a bacterial transporter
, and additional modulator mutations can finetune specificity when combined with core-position mutations. The modulator mutations overlap considerably with key epistatic mutations, and we use this insight to propose a new biochemical model for how mutations that alter conformational balance in transporters can lead both to long-range epistasis and specificity modulation.
Authors
- Samuel P. Berry (ORCID: https://orcid.org/0000-0002-3480-5988)
- Debora S. Marks (ORCID: https://orcid.org/0000-0001-9388-2281)
- Camille B. Freedman
- Rachelle Gaudet (ORCID: https://orcid.org/0000-0002-9177-054X)
Institutions
- Broad Institute (US)
- Harvard University (US)
- Harvard University Press (US)
- Harvard Bioscience (United States) (US)
- Center for Systems Biology (US)
- Artificial Intelligence in Medicine (Canada) (CA)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2026-03-31
- DOI
- https://doi.org/10.64898/2026.03.30.714904
- Primary Topic
- Bacterial Genetics and Biotechnology
- Type
- article
- Field-Weighted Citation Impact
- 0.00