Evaluating Anticancer Activity of Karveeradi Yoga in Cervical Cancer Cell Lines: An In-vitro Study Research Protocol

Introduction: Ayurveda, a traditional Indian system of medicine, emphasises the use of natural remedies. Karveeradi Yoga, derived from classical Ayurvedic scriptures, consists of potent herbs such as Nerium indicum and Moringa oleifera, which have traditionally been used for detoxification and for treating conditions resembling cancer. Cervical cancer, a significant global health burden, arises from persistent Human Papillomavirus (HPV) infections and necessitates the exploration of effective alternative treatment options. Need of the study: The rising incidence of cervical cancer highlights the limitations of conventional therapies, including their high toxicity, cost, and adverse side-effects. In view of these challenges, exploring the anticancer potential of Ayurvedic formulations such as Karveeradi Yoga may offer safer and more affordable therapeutic alternatives. Aim: To standardise Karveeradi Yoga and evaluate its anticancer activity in cervical cancer cell lines. Materials and Methods: An in-vitro experimental preclinical study will be conducted from June 2025 to December 2025 at the Biocyte Institute of Research and Development, Sangli, Maharashtra India, in collaboration with the Department of Agada Tantra, Mahatma Gandhi Ayurved College and Hospital and Research Centre, Wardha, Maharashtra, India. Raw materials will be authenticated, standardised, and prepared according to Bhavprakash Samhita. Physicochemical and phytochemical analyses will be performed. Two cervical cancer cell lines, HeLa and SiHa, will be subjected to MTT assay, flow cytometry, and IC50 determination. Positive controls (standard chemotherapy agents) and vehicle controls will be included. Statistical analysis will be performed using Analysis of Variance (ANOVA) or t-tests, with p-value <0.05 considered statistically significant.