Genome Integrity in Dairy Cows Fed Black Soldier Fly Oil: An Integrated Sister Chromatid Exchange and Alkaline Comet In Vivo Assessment
BACKGROUND/OBJECTIVES: = 13) for 50 days. METHODS: Peripheral blood lymphocytes were analyzed using Sister Chromatid Exchanges (SCE), reflecting replication-associated chromosomal instability, and the alkaline Comet assay, quantifying primary DNA damage at the single-cell level (Tail DNA and Olive tail moment) at T0 (the day before the start of the two experimental diets), T1 (30 d) and T2 (50 d). RESULTS: = 0.017). CONCLUSIONS: Overall, no evidence of genotoxicity was observed in cows fed HIO; conversely, the results support compatibility with genome stability and suggest a modest time-dependent improvement detectable mainly after prolonged supplementation.
Authors
- Cristina Rossetti (ORCID: https://orcid.org/0000-0003-1944-0068)
- Alfredo Pauciullo (ORCID: https://orcid.org/0000-0002-3140-9373)
- V. Genualdo (ORCID: https://orcid.org/0000-0001-7635-4508)
- Mathieu Gerbelle
- Giustino Gaspa (ORCID: https://orcid.org/0000-0003-1604-8917)
- A. Perucatti (ORCID: https://orcid.org/0000-0003-4642-9921)
- Lara Rastello (ORCID: https://orcid.org/0000-0001-5185-2797)
- Giulia Milanese
- Martina Alessandra Gini
- Manuela Renna
- Flavia Caserta
- Laura Gasco
Institutions
- Agenzia Regionale per la Protezione Ambientale del Piemonte (IT)
- Istituto per il Sistema Produzione Animale in Ambiente Mediterraneo (IT)
- International Centre for Advanced Mediterranean Agronomic Studies (IT)
- Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta (IT)
- University of Turin (IT)
Publication Details
- Journal
- Genes
- Published
- 2026-03-31
- DOI
- https://doi.org/10.3390/genes17040404
- Primary Topic
- Insect Utilization and Effects
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Ministeriet for Fø devarer, Landbrug og Fiskeri
- Agentschap Innoveren en Ondernemen
- Vlaamse regering
- Grønt Udviklings- og Demonstrations Program