DNA/BSA Binding, Antioxidant, and Enzyme Inhibition Studies of Azomethine‐Based Complexes of Essential Bioelements

ABSTRACT Since time immemorial, azomethine‐based coordination compounds have been extensively explored toward various applications. Such complexes have perfect synergism, thereby exerting improved biological efficacy and therapeutic function. In the current study, we have synthesized a hitherto unreported ligand (H2L) from 4‐Fluoro‐o‐phenylenediamine and 2‐Hydroxy‐4‐methoxybenzaldehyde and its corresponding copper, zinc, and nickel complexes. The compounds are characterized by UV‐Visible, FTIR, NMR, EPR, and HRMS. Further, we have investigated the binding propensities of the complexes with DNA and BSA through UV absorption titration and fluorescence quenching assays. The interaction with DNA provides insight into the anticancer potential of the designed compounds. Based on UV titration of the complexes with DNA, it has been observed that the copper complex (CuL) has a higher binding constant value (K b = 6.43 ×10 5 M −1 ) than other complexes. The binding potential of the compounds with BSA throws light on drug transport and distribution in the biological system, as it acts as a drug carrier. As per fluorescence assay of the compounds with BSA, the nickel complex (NiL) has a higher quenching constant value (K q = 6.92 ×10 12 M −1 ) than other complexes. Further, the DNA cleaving abilities, antioxidant, and enzyme inhibitory potential of the complexes are assessed.