<i>Pseudomonas aeruginosa</i> exoenzyme Y activity inhibits exoenzyme S-induced caspase activation in pulmonary microvascular endothelial cells
strain PAK utilizes a type III secretion system to inject exoenzymes S, T, and Y directly into the host cell cytosol. In pulmonary microvascular endothelial cells, exoenzyme S (ExoS) alone, but not exoenzymes T or Y (ExoT or ExoY) alone, induces apoptotic caspase activation and cell death. When exoenzymes are coinjected, ExoY, but not ExoT, inhibits ExoS-induced caspase activation, but not cell death. ExoY must be catalytically active to prevent ExoS-induced caspase activation.
Authors
- Phoibe Renema (ORCID: https://orcid.org/0000-0003-0230-7068)
- Terrence J. Ravine (ORCID: https://orcid.org/0000-0003-0814-6306)
- Connor Holm
- Troy Stevens (ORCID: https://orcid.org/0000-0001-9689-929X)
- Jonathon P. Audia (ORCID: https://orcid.org/0000-0002-0022-4800)
- Sarah Sayner (ORCID: https://orcid.org/0000-0002-4587-6634)
- Magan N. Pittman
- Alana Smith-Gorcey
- Andrea Vavrinek
- Madison Gray
- Katlyn Bouler
- Hoa Tran
Institutions
- University of South Alabama (US)
Publication Details
- Journal
- American Journal of Physiology-Lung Cellular and Molecular Physiology
- Published
- 2026-04-01
- DOI
- https://doi.org/10.1152/ajplung.00373.2025
- Primary Topic
- Bacterial biofilms and quorum sensing
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- University of South Alabama
- American Society for Biochemistry and Molecular Biology