HIV Promoters Isolated from Brain and Peripheral Tissue of Virally Suppressed PWH Are Phylogenetically and Functionally Similar
= 5). Following single-genome amplification, sequences were phylogenetically analyzed and transcriptional activity was assessed. In contrast to non-virally suppressed PWH, LTR sequences failed to compartmentalize between the brain and peripheral compartments. Identical LTR sequences were observed across brain and peripheral tissues in 2/5 PWH. While the LTRs remain transcriptionally active, mutations, insertions and deletions predicted to reduce transcription factor binding affinity at key binding sites, including C/EBP, NF-κB, and Sp1 sites, were observed and found to result in reduced basal transcriptional activity. The role of these mutations in latency and viral persistence remains unclear.
Authors
- Anna C. Hearps (ORCID: https://orcid.org/0000-0002-8492-9815)
- Narin Osman (ORCID: https://orcid.org/0000-0002-6738-6513)
- Janna Jamal Eddine (ORCID: https://orcid.org/0000-0002-2087-2717)
- Jingling Zhou (ORCID: https://orcid.org/0000-0002-2195-6314)
- Sarah J. Byrnes (ORCID: https://orcid.org/0000-0002-3580-8574)
- Thomas A. Angelovich (ORCID: https://orcid.org/0000-0003-2069-0451)
- Trisha A. Jenkins (ORCID: https://orcid.org/0000-0003-0137-6643)
- Melissa J. Churchill (ORCID: https://orcid.org/0000-0001-5510-7954)
- Emily Chalmers
- Michael Roche
Institutions
- Burnet Institute (AU)
- Peter Doherty Institute (AU)
- Monash University (AU)
- RMIT University (AU)
Publication Details
- Journal
- International Journal of Molecular Sciences
- Published
- 2026-03-31
- DOI
- https://doi.org/10.3390/ijms27073185
- Primary Topic
- HIV Research and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Health and Medical Research Council
- National Institute on Drug Abuse