Saparation and identification of bioactive compounds from the seeds of Iraqi physalis angulata L. using chromatographic techniques

This study aimed to investigate the phytochemical composition of Physalis angulata L collected from the Rabia region, west of Mosul, Nineveh Governorate/ Iraq. The active compounds of selected P. angulata seeds were identificated sued chromatographic techniques. The extraction of those active compounds was performed employing the Soxhlet apparatus. The results showed the identification of six phenolic compounds in the 70% alcoholic extract by High-Performance Liquid Chromatography (HPLC) analysis following acid hydrolysis (Quercetin, Apigenin, Ferulic acid, P-coumaric acid, Gallic acid, and Chlorogenic acid). And the Chlorogenic acid was exhibited the highest concentration in the extract reaching 0.0904 mg\ml. Fourteen compounds were identified in the same crude extract used Gas Chromatography-Mass Spectrometry (GC- MS)analysis, among which the most prominent were (17-Octadecynoic acid, 3-Hexanol, 1,2-Benzenedicarboxylic acid, Heptanoic acid, 2-ethyl, and Lauric aacid) Gas-liquid chromatography (GLC) analysis of the saponified petroleum ether extract revealed presence of six fatty acids (Oleic, Stearic, Linoleic, Palmatic, Arachidonic, Linolenic). Among these, Linoleic acid was the most abundant, constituting 19.80% of the extract. These results indicate that the seeds of P. angulata contain compounds of medical importance, which opens the door to its potential use in future therapeutic pharmaceutical applications.