Rapid Detection of Carbapenem-Producing and Enzyme Category in <i>Enterobacterales</i> through Ultraviolet Spectroscopy-Based Meropenem Hydrolysis Assessment
. The assay demonstrated 98.54% accuracy in detecting Meropenem resistance using an 85% Meropenem retention threshold, and both the hydrolysis and inhibitor enhancement assays showed 97.47% specificity for distinguishing MBL from non-MBL producers. In conclusion, this UV spectrophotometry-based assay provides a rapid, accurate, and economical approach for detecting carbapenem resistance and classifying carbapenemase type, offering great potential for improving CPE infection management and guiding antibiotic therapy.
Authors
- Jiaxin Tang (ORCID: https://orcid.org/0000-0002-7210-5405)
- Jiacheng Ding (ORCID: https://orcid.org/0000-0002-8732-5339)
- Yan-Dan Li
- Li-Ting Dai
- Hui-min Zhang
- Fei-qi Chen
- Ding-qiang Chen
- Qi-hui Zhan
- Meng-xue Zou
Institutions
- First Affiliated Hospital of Guangzhou Medical University (CN)
- Zhujiang Hospital (CN)
- State Key Laboratory of Respiratory Disease (CN)
- Southern Medical University (CN)
- Guangzhou Medical University (CN)
Publication Details
- Journal
- ACS Infectious Diseases
- Published
- 2026-04-01
- DOI
- https://doi.org/10.1021/acsinfecdis.5c00794
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- China Primary Health Care Foundation
- Special Fund Project for Science and Technology Innovation Strategy of Guangdong Province