Ozone Therapy Modulates Histopathological and Biochemical Markers in Mice with Induced Polycystic Ovary Syndrome

Background — Polycystic ovary syndrome (PCOS) is an endocrine disorder characterized by polycystic ovaries and associated oxidative stress. This study assessed the potential beneficial effects of ozone therapy on ovarian tissue in mice with induced PCOS. Methods — Forty-two female mice were distributed between six groups: a control group, a PCOS group, a PCOS group treated with ozone (45, 60, and 70 μg/mL), and a PCOS group treated with clomiphene citrate (1.5 mg/kg). PCOS was induced by injection of estradiol valerate (EV), and treatment was administered for four weeks. Data were analyzed using SPSS software, with a significance level of p