Multi-strain analysis of <i>Pseudomonas putida</i> reveals the metabolic and genetic diversity of the species
strains using a combination of genome sequencing, phenotypic profiling, and metabolic modeling. Our results indicate that while many metabolic pathways are conserved, notable differences exist across strains, particularly in aromatic compound degradation. These observations may inform future strain selection and engineering strategies tailored to specific industrial or environmental goals. In addition, the genome-scale models and phenotypic data generated here can serve as a foundation for broader studies of metabolism and functional variation within this species.
Authors
- Anthony X. Ayala (ORCID: https://orcid.org/0000-0002-3913-6453)
- Wei Niu (ORCID: https://orcid.org/0000-0003-3826-1276)
- Qixing Wei (ORCID: https://orcid.org/0000-0003-4393-7074)
- Hans Verkler
- Adam M. Feist (ORCID: https://orcid.org/0000-0002-8630-4800)
- Ying Hefner (ORCID: https://orcid.org/0000-0003-3092-2914)
- Jonathan M. Monk (ORCID: https://orcid.org/0000-0002-3895-8949)
- Jayanth Krishnan (ORCID: https://orcid.org/0000-0001-5199-6387)
- Joshua Mueller (ORCID: https://orcid.org/0000-0001-9092-1222)
- Bernhard O Palsson
- Juan D. Tibocha-Bonilla
Institutions
- University of Nebraska–Lincoln (US)
- University of California San Diego (US)
- La Jolla Bioengineering Institute (US)
- Joint BioEnergy Institute (US)
- University of Puerto Rico-Mayaguez (PR)
- Technical University of Denmark (DK)
Publication Details
- Journal
- mSystems
- Published
- 2026-04-01
- DOI
- https://doi.org/10.1128/msystems.01594-25
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- U.S. Department of Energy
- U.S. Department of Agriculture
- Nebraska Center for Energy Sciences Research, University of Nebraska-Lincoln
- Novo Nordisk Fonden
- National Science Foundation of Sri Lanka
- National Institute of General Medical Sciences