Utilizing a culture system for horizontal cells to study neural circuit assembly in the developing mouse retina
. Moreover, neurite extension of horizontal cells is limited to early developmental stages as young mice extend more complex processes compared to those from adolescent retinas. We also found that horizontal cells, unlike retinal ganglion cells, do not extend neurites when cultured alone and require other retinal neurons to promote neurite outgrowth. In summary, we established a horizontal cell culture system that can be used to decipher the mechanisms involved in neural circuit assembly of the mouse retina.
Authors
- Arlene A. Hirano
- Marlon Fraga Mattos (ORCID: https://orcid.org/0000-0001-7795-6543)
- Yong Hwan Park
- Ross Perez
- Rajashree Venkatraman
- Rinki Ratnapriya
- Elizabeth Zuniga-Sanchez
- Benjamin J. Frankfort (ORCID: https://orcid.org/0000-0001-8096-9812)
- Daniela Becerril
- Nicholas C. Brecha
- Collin Todora
Institutions
- University of California, Los Angeles (US)
- Baylor College of Medicine (US)
- VA Greater Los Angeles Healthcare System (US)
Publication Details
- Journal
- Frontiers in Cellular Neuroscience
- Published
- 2026-04-01
- DOI
- https://doi.org/10.3389/fncel.2026.1691122
- Primary Topic
- Retinal Development and Disorders
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Research to Prevent Blindness
- BrightFocus Foundation
- Genentech