Synthesis of Bio-Based Polyamide 6,5 via Enzymatic Polycondensation

This work aimed to explore novel polymeric material by synthesizing polyamide 6,5 via the direct enzymatic polycondensation of dimethyl glutarate and 1,6-Diaminohexane, using the lipase Novozym 435 as a biocatalyst. While maintaining a fixed monomer feed ratio, the effects of reaction temperature, duration, and enzyme concentrations on the molecular weight and yield of the resulting polyamide 6,5 were systematically investigated. The experimental results indicated that the optimal conditions for the Novozym 435-catalyzed synthesis were a reaction time of 3 days, a temperature of 90 °C, and enzyme concentrations of 20 wt%. The establishment of this enzymatic synthesis route for polyamide 6,5 not only provides a novel methodology for polymer synthesis but also offers a new perspective for the future green materials manufacturing industry.