Digital PCR as a potential reference measurement procedure to support monkeypox virus/Orthopoxvirus external quality assessment schemes
copies/mL. The candidate reference method was applied to clinical MPXV patients' samples. The estimated viral DNA quantity in clinical samples using quantitative real time PCR (qPCR) showed good correlation and agreement with dPCR reference value. Thus, the dPCR-based method enabled reliable value assignment of MPXV reference materials. This method offers a calibration-free alternative for labs without access to certified standards. Our findings suggest that dPCR could be used for reference measurement value assignment of MPXV reference materials to support calibration of MPXV viral load testing in molecular diagnostic testing laboratories.
Authors
- Martin Kammel (ORCID: https://orcid.org/0000-0002-2011-086X)
- Alexander Hübner (ORCID: https://orcid.org/0000-0003-3572-9996)
- Esmeralda Valiente (ORCID: https://orcid.org/0000-0002-5262-4697)
- Ingo Schellenberg
- Robert Ehret (ORCID: https://orcid.org/0000-0001-6960-0141)
- A. Kummrow (ORCID: https://orcid.org/0000-0002-2536-1377)
- Heinz Zeichhardt (ORCID: https://orcid.org/0000-0001-9571-8995)
- Samreen Falak (ORCID: https://orcid.org/0000-0002-8454-7884)
- Ulf Dühring
- M Obermeier
- Jörn Beheim-Schwarzbach
- Hans‐Peter Grunert
- Annemarie Martin
- Alina Groß
Institutions
- Physikalisch-Technische Bundesanstalt (DE)
- Gesellschaft für Klinische Forschung (DE)
- Deutsches Herzzentrum der Charité (DE)
- BQS Institut für Qualität und Patientensicherheit (Germany) (DE)
- Deutsche Gesellschaft für Urologie (DE)
Publication Details
- Journal
- Methods
- Published
- 2026-03-31
- DOI
- https://doi.org/10.1016/j.ymeth.2026.03.013
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- European Association of National Metrology Institutes