Advanced physiological maturation of human iPSC-derived cardiomyocytes using an algorithm-directed optimization of defined media components
handling, electrophysiology, and metabolism, which was further validated by multi-omic screening, for cells in either pure or co-cultured microtissue formats. Together, these findings not only provide a reliable workflow for highly functional hiPSC-CMs for downstream use, but also demonstrate the power of high-dimensional optimization processes in evoking advanced biological function in vitro.
Authors
- WENLIANG CHEN
- Neal I. Callaghan (ORCID: https://orcid.org/0000-0001-8214-3395)
- J. Paul Santerre (ORCID: https://orcid.org/0000-0003-3373-6463)
- Julie Audet (ORCID: https://orcid.org/0000-0002-5599-6074)
- Ronald G. Ireland (ORCID: https://orcid.org/0000-0002-4464-5412)
- Cristine J. Reitz (ORCID: https://orcid.org/0000-0002-1748-0501)
- Renée A. Gorman (ORCID: https://orcid.org/0009-0006-9580-9807)
- Anthony O. Gramolini (ORCID: https://orcid.org/0000-0003-1109-2070)
- Lauren J. Durland (ORCID: https://orcid.org/0000-0001-5740-8670)
- Craig A. Simmons (ORCID: https://orcid.org/0000-0001-7729-1772)
- Karl T. Wagner (ORCID: https://orcid.org/0000-0002-5501-7622)
- Erika Yan Wang (ORCID: https://orcid.org/0000-0002-1634-7493)
- Uroš Kuzmanov (ORCID: https://orcid.org/0000-0002-3502-6986)
- Maria Zena Miranda (ORCID: https://orcid.org/0000-0001-5354-2485)
- Filio Billia
- Seema Mital
- Milica Radisic
- James Ellis
- YU LAI DING
- Zahra Mirzaei
- Peter H. Backx
- Michelle M. Kim
Institutions
- Dalhousie University (CA)
- University Health Network (CA)
- Guangdong Medical College (CN)
- University of Toronto (CA)
- York University (CA)
- Hospital for Sick Children (CA)
- Toronto General Hospital (CA)
- Ted Rogers Centre for Heart Research (CA)
Publication Details
- Journal
- Nature Communications
- Published
- 2026-03-31
- DOI
- https://doi.org/10.1038/s41467-026-70550-9
- Primary Topic
- Cardiac Fibrosis and Remodeling
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Canadian Institutes of Health Research
- Natural Sciences and Engineering Research Council of Canada