Systematic Evaluation of Maleimide Spacer Impact on Drug-Linker Deconjugation in Antibody-Drug Conjugates <i>Ex Vivo</i> and <i>In Vivo</i>
) and ADC (conjugated antibody) ELISA pharmacokinetic (PK) profiles. While deconjugation profiles were generally consistent across methods, IP-MS provided more quantitative measurements and was more sensitive to subtle differences in deconjugation. Our findings shed light on the importance of the spacer structure on thiol-maleimide ADC stability.
Authors
- Raffaele Colombo (ORCID: https://orcid.org/0000-0001-5709-4704)
- Jodi Wong (ORCID: https://orcid.org/0009-0004-8505-2690)
- Kaylee J. Wu (ORCID: https://orcid.org/0009-0009-6827-5960)
- D.A. Alonzo (ORCID: https://orcid.org/0000-0002-3586-3485)
- Andrea Hernández Rojas (ORCID: https://orcid.org/0000-0002-0278-9434)
- Graham A. E. Garnett (ORCID: https://orcid.org/0009-0008-5995-8308)
- Vincent Fung (ORCID: https://orcid.org/0009-0003-8513-0591)
- Stuart D. Barnscher (ORCID: https://orcid.org/0009-0000-2386-5240)
- Luying Yang (ORCID: https://orcid.org/0009-0002-3732-2364)
- Ambroise Wu (ORCID: https://orcid.org/0000-0001-8568-8331)
- Tong Ding
- Kevin Yin
- Mark E. Petersen
- Samir Das
Institutions
- Zymeworks (Canada) (CA)
Publication Details
- Journal
- Bioconjugate Chemistry
- Published
- 2026-03-31
- DOI
- https://doi.org/10.1021/acs.bioconjchem.5c00580
- Primary Topic
- HER2/EGFR in Cancer Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00