Development of One-Tube Multiplex Arbitrary (RAPD and ISSR) Marker-Based SCAR Assay for Simultaneous Detection and Authentication of Indian Senna (Senna alexandrina Mill.) and Its Adulterant Species

. The multiplex amplification profiles were entirely consistent with corresponding uniplex assays, endorsing the method's robustness and reproducibility. This streamlined, one-tube multiplex SCAR-PCR system represents a significant advancement toward reliable, high-throughput molecular authentication of Indian senna and its closely related medicinal plant species (adulterants).