Cs2CO3-mediated facile synthesis, characterizations, and biological activities of 4H-benzo[4,5]thiazolo[3,2-a]pyrimidin-4-ylidene)acetonitrile derivatives

In this study, we report the newer method for the synthesis of 4H-benzo[4,5]thiazolo[3,2-a]pyrimidin-4-ylidene) acetonitrile and the biological activities of the derivatives were systematically evaluated. The antimicrobial potential of the compounds was assessed against three bacterial and three fungal strains using the agar diffusion method. Among the derivatives tested, compounds 3b, 3e, and 3h demonstrated notable antibacterial and antifungal activities. Furthermore, the antioxidant capacity of the selected compounds was investigated through the DPPH radical scavenging assay. Compounds 3e and 3f exhibited significant radical scavenging activity, achieving effective inhibition at a concentration of 0.1 mg/mL. These findings highlight the promising antimicrobial and antioxidant properties of the investigated thiazolopyrimidine derivatives and support their potential for further biological and pharmacological studies.