Effect of AG1® supplementation on nutritional adequacy and gut microbial composition in trained adults

Background: Dietary supplements that combine vitamins, minerals, phytonutrients, prebiotics, and probiotics have gained popularity among health-oriented consumers seeking convenient ways to fill nutritional gaps and support gut health. Methods: or placebo (PL) for 14 days. Following a 2-week washout, participants crossed over to the other condition. Participants provided stool samples for gut microbial composition analysis, completed the Digestion-associated Quality of Life Questionnaire (DQLQ), and completed a 24-h dietary intake assessment at the beginning and end of each 14-day supplementation period. Outcomes were analyzed using repeated-measures and multivariate statistical approaches for dietary intake, gut microbiota, metabolomics, and questionnaire data. Results: = 0.777). Vitamins A, C, and E were the most common nutrient gaps filled by AG1 supplementation. Conclusions: was well tolerated and did not negatively impact digestion-associated quality of life. Clinical trial registration: ClinicalTrials.gov, identifier: NCT06521424.