Preventing peri-implantitis with Ag-Est816 nanocomposite: modified rat model and preventive assessment

Background: Peri-implantitis is a prevalent complication leading to progressive bone loss around dental implants, with current therapeutic strategies offering limited preventive efficacy. Objective: This study aimed to develop a modified rat model of peri-implantitis to evaluate the effects of a silver nanoparticle and quorum quenching enzyme nanocomposite (Ag-Est816) on peri-implant bone health. Methods: were surgically placed into the femurs of five male Sprague-Dawley rats weighing 180-220 g. After four weeks, peri-implant bone loss was evaluated using micro-computed tomography (Micro-CT) to measure bone volume to total volume ratio (BV/TV), and histological analysis was performed to assess bone architecture. Non-infected implants served as controls. In Part B, Ag-Est816 was applied to infected titanium implants, while control implants were treated with sterilized phosphate-buffered saline (PBS). Micro-CT and histological analysis were used to evaluate bone loss and tissue integrity. Results: < 0.001). Histological analysis revealed new bone regeneration with trabecular integrity around Ag-Est816-treated implants, in contrast to compromised osseointegration with disrupted, sparse bone and fibrous, inflammatory tissue in the controls. Conclusions: A modified rat femur model of peri-implantitis was successfully developed. Ag-Est816 effectively prevented peri-implant bone loss.