INVITRO ANTIDIABETIC STUDY AND PHYTOCHEMICAL SCREENING OF HYDROALCOHOLIC EXTRACT OF ROTHECA MICROPHYLLA (BLUME) CALLM. & PHILLIPSON LEAVES

Diabetes mellitus is a collection of metabolic disorders that are characterized by persistently high blood sugar levels. The study focuses on the invitro anti diabetic study and phytochemical screening of hydroalcoholic extract of Rotheca microphylla (Blume) Callm. & Phillipson leaves. Herbal medicines have gained increasing attention due to their therapeutic potential, safety profile, affordability and cultural acceptance. The study aims to provide scientific evidence for the traditional use of Rotheca microphylla and to explore it's potential as a natural Source of anti-diabetic agents. The study includes plant collection, Authentication, shade- drying, and subjected to hydroalcoholic Soxhlet extraction. Pharmacognostic parameters such as stomatal number, stomatal index, vein islet number, and vein termination number were assessed for proper identification and standardization. Preliminary phytochemical screening of the crude extract revealed the presence of bioactive constituents including flavonoids, phenolics, alkaloids, tannins, saponins, terpenoids, glycosides, and carbohydrates. The antidiabetic activity was confirmed through in-vitro enzyme inhibition assays, specifically α- amylase and α-glucosidase inhibition methods, which are key targets in carbohydrate metabolism and post-prandial glucose regulation. Findings from phytochemical and enzyme inhibition analyses suggest that the plant possesses promising bioactive compounds that may contribute to glucose-lowering effects, thereby supporting further pharmacological and clinical investigations.