Chromosome-Level Genome Assembly and Annotation of Piptanthus nepalensis (Hook.) Sweet

Piptanthus nepalensis (Hook.) Sweet is a leguminous shrub native to the Himalayan region and is valued for its medicinal uses and ornamental yellow flowers. Here, we report a chromosome-scale genome assembly of P. nepalensis generated using PacBio HiFi long reads, Illumina short reads, and Hi-C chromatin interaction data. The assembled genome spans approximately 1.04 Gb, with a contig N50 of 39.5 Mb and a scaffold N50 of 111.5 Mb. Benchmarking universal single-copy orthologs (BUSCO) analysis indicated high completeness for both the genome (99.3%) and predicted protein set (99.2%). Approximately 99.0% of the assembled sequences were anchored onto nine pseudo-chromosomes. We annotated 26,035 protein-coding genes, and repetitive elements were estimated to comprise ~75.2% of the genome. This high-quality genome assembly provides a foundational genomic resource for P. nepalensis and supports future studies on its biology and utilization.