Intestinal Anti-inflammatory and Antioxidant Properties of Moringa oleifera Alkaloids in an Experimental Model of IBD

Introduction: Inflammatory bowel disease (IBD) is a chronic intestinal disorder that affects many individuals worldwide. Moringa oleifera is a valued plant offering sig-nificant therapeutic and nutritional benefits. It has emerged as a promising source of bio-active compounds. Among its phytochemicals, alkaloids are known for their notable anti-oxidant and anti-inflammatory activities. This study aims to investigate the potential anti-oxidant and anti-inflammatory effects of a total alkaloid extract from Moringa oleifera leaves (AMO) in a DNBS-induced colitis model in BALB/c mice. Methods: Three doses of total alkaloid extract from Moringa oleifera (AMO) (25, 50, and 100 mg/kg) were administered orally after the induction of colitis via rectal administration of DNBS. The inflammation was recorded daily using the disease activity index (DAI), biochemical analysis, and histological assessment. In this context, colon tissue samples were used to assess microscopic scores and to determine the dosage of biochemical mark-ers, including myeloperoxidase (MPO) and catalase (CAT) activities, and reduced gluta-thione (GSH), malondialdehyde (MDA), and nitric oxide (NO) levels. Results: It was determined that AMO treatment significantly reduced the severity of colitis, evidenced by reduced DAI and lower histological damage scores. Antioxidant markers were elevated in the treated groups, whereas NO, MDA, and MPO levels were elevated in untreated colitic mice. Discussion: The results highlight the therapeutic potential of Moringa oleifera alkaloid extract; the anti-inflammatory effect may be mediated by reductions in MDA, MPO, and NO levels and by increases in CAT and GSH levels. conclusion: The results highlight the therapeutic potential of Moringa oleifera alkaloid extracts in mitigating intestinal inflammation and oxidative stress. These position Moringa oleifera as a promising candidate in the management of IBD and enhancing gut health. Conclusion: These findings suggest that Moringa oleifera alkaloid extract may offer a promising natural adjunct for IBD management through modulation of oxidative and in-flammatory pathways.