Chromosome-level assembly of spotted steed (Hemibarbus maculatus Bleeker, 1871) genome

Spotted steed (Hemibarbus maculatus Bleeker, 1871), a small and medium-sized benthic fish, is widely distributed between the Yangtze and Heilongjiang River basins and is considered to be one of the most widely distributed freshwater fish in East Asia. It is also an economically valuable aquaculture fish and has become a commercial freshwater aquaculture species in China. Here, a high-quality chromosome-level genome of spotted steed was produced by combining PacBio single molecule sequencing technique and high-throughput chromosome conformation capture technologies. Ultimately, the genome was assembled into 1098.66 Mb with a contig N50 of 33.57 Mb and a scaffold N50 of 40.40 Mb. We constructed a chromosome-level genome assembled with 25 chromosomes, whose total lengths accounted for 97.49%, and the assembled genome represents 95.64% completeness (BUSCO). We also identified 285.16 Mb (25.96%) of repetitive genome sequences and 23233 predicted genes. A total of 23021 genes were functionally annotated, representing 99.09% of the predicted genes. These results will provide valuable genomic resources for subsequent study of the genetic, evolutionary, and biological characteristics of spotted steed.