The azole-resistance phenotype of a <i>Nakaseomyces glabratus</i> clinical strain encoding a wild-type <i>PDR1</i> allele involves the efflux pumps Aus1 and Pdh1 and Cyb5, an alternative reductase required for ergosterol biosynthesis

The emergence of resistance to azoles among strains of <i>Nakaseomyces glabratus</i> is a major cause of concern, considering the importance of this yeast in causing human infections. In this work, we disclose a set of genes that mediate the azole-resistance phenotype of a resistant clinical strain, with one of these players (Cyb5) being also implicated in biosynthesis of ergosterol for the first time. With this, we increase a set of possible therapeutic targets that can be used in future studies to develop new antifungals.