适配体夹心免疫传感器在生物检测中的应用

Abstract Aptamer-based sandwich immunosensors are an emerging analytical platform that combines the high specificity recognition of aptamers with the high sensitivity of immunoassays.Due to their advantages of excellent selectivity and low detection limits these biosensors hold great potential for use in bioanalytical applications.This review outlines the key strategies underlying such biosensors including aptamer selection sandwich structure construction and nanomaterial-enhanced signal amplification and surveys recent advances in their bioanalytical application for the detection of small molecules proteins and pathogenic microorganisms.Current challenges and promising future directions for the field are also discussed.Keywords aptamer nanomaterials sandwich immunosensor bioanalysis 1