Family single-cell atlases reveal pig pregnancy and fetal growth restriction critical cell types
Although massive cell atlases are already available, it is still a challenge to obtain an atlas of all tissues within a single body, especially in fetuses and pregnant mothers. We present a transcriptomic atlas of 2.56 million single cells covering 115 and 119 tissues from one fetal pig and its pregnant mother, respectively. We found that a cluster of heart capillary endothelial cells with enhanced fatty acid transit capability was enriched for pregnancy but restored after gestation. We also deciphered that l-leucine transport insufficiency in the trophoblast causes fetal growth restriction by reducing a muscle type II myofiber subcluster. Our "all-from-one" strategy enabled the identification of tissue cell type-specific transcription factors and provided insights into pregnancy heart adaptation and fetal growth restriction.
Authors
- Yaxiang Wang (ORCID: https://orcid.org/0000-0001-6919-7129)
- Chuanmin Qiao (ORCID: https://orcid.org/0000-0002-1029-5936)
- Jun Yang (ORCID: https://orcid.org/0000-0003-3420-2543)
- Zifeng Liu (ORCID: https://orcid.org/0000-0003-4811-8799)
- Ziqi Ling (ORCID: https://orcid.org/0000-0001-5655-6495)
- Haoyun Jiang
- Chao Wei
- Shuqi Xiong
- Cong Huang (ORCID: https://orcid.org/0000-0001-6149-4989)
- Jianjun Li (ORCID: https://orcid.org/0009-0003-2777-1796)
- Xiao Sun (ORCID: https://orcid.org/0000-0002-6860-747X)
- Chenyu Li (ORCID: https://orcid.org/0000-0002-8037-3098)
- D.-Q. Cui
- C. Y. Chen
- Yunyan Zhou (ORCID: https://orcid.org/0000-0002-6642-1428)
- Zuoquan Chen (ORCID: https://orcid.org/0000-0002-5116-7746)
- Xinke Xie
- Weiwei Zhou (ORCID: https://orcid.org/0000-0003-1641-8965)
- Shuai Lin
- Qiqi Jing (ORCID: https://orcid.org/0000-0003-0567-3506)
- Yu Ye (ORCID: https://orcid.org/0000-0002-6621-6694)
- Lusheng Huang (ORCID: https://orcid.org/0000-0002-6940-667X)
- Qiang Yang (ORCID: https://orcid.org/0000-0002-9890-263X)
- M. H. Liu
- Siguang Li (ORCID: https://orcid.org/0000-0002-1740-2060)
- Jianzhen Huang (ORCID: https://orcid.org/0000-0001-8086-1860)
- Hao Fu (ORCID: https://orcid.org/0000-0001-7576-0942)
- Yiwen Xiahou
- Jing Li (ORCID: https://orcid.org/0000-0001-9392-6600)
- Qing Zhang (ORCID: https://orcid.org/0000-0002-1160-2715)
- Weiwei Liu (ORCID: https://orcid.org/0000-0001-6997-2412)
- Li Li (ORCID: https://orcid.org/0000-0003-3966-6363)
- Zhe Chen (ORCID: https://orcid.org/0000-0003-2611-9635)
- Chuangang Yu
- Jinyuan Wu
- S Samuel Yang (ORCID: https://orcid.org/0000-0002-7911-8444)
- Liqing Chen (ORCID: https://orcid.org/0000-0002-4857-2097)
- Siyi Liu (ORCID: https://orcid.org/0000-0001-6852-0684)
- Lei Xie (ORCID: https://orcid.org/0009-0005-2191-0999)
- Jianchao Hu
- Mengqing Zhou (ORCID: https://orcid.org/0000-0003-2831-9673)
- Hui Jiang (ORCID: https://orcid.org/0000-0001-8393-8368)
- Fusheng Wu
- Mei Ge (ORCID: https://orcid.org/0000-0002-3094-8877)
- Min Yan (ORCID: https://orcid.org/0009-0005-7070-0250)
- Xi Tang (ORCID: https://orcid.org/0009-0006-7235-5099)
- Jingquan Li
- Lin Wu (ORCID: https://orcid.org/0000-0003-0239-4456)
- Zhang Hu (ORCID: https://orcid.org/0009-0008-7876-7654)
- Yaya Liao
- Yujie Shu (ORCID: https://orcid.org/0009-0007-5083-8425)
- Yü Huang (ORCID: https://orcid.org/0000-0002-1277-6784)
- Naixiang Yu (ORCID: https://orcid.org/0009-0006-8767-1991)
- Xianhua Xie
- Huanfa Gong (ORCID: https://orcid.org/0000-0002-4606-0151)
- Guanyue Wei
- Xiaoyun Chen (ORCID: https://orcid.org/0009-0004-6778-9787)
- Yingchun Sun
- Min Zheng (ORCID: https://orcid.org/0000-0002-3319-5647)
- Yuxin Liu (ORCID: https://orcid.org/0009-0000-6987-8741)
- Xiaofen Hu
- Yanyuan Xiao
- Tianxiong Yao
- Huashui Ai
- Jiahong Zhong
- Xiaodong Liu
- Chao Yin
- Liping Cai
- Yuyun Xing
- Qingjie Zeng
- Zhong Wang
- Qin Liu
- Tao Shen
- Sha Liu
- Zhou Zhang
- Yong Hou
- Bin Yang
- Sanya Xiong
- Feng Chen
- Xiaolong Chang
- Zhiwei Peng
- Shijun Xiao
- Jun Gao
- Hui Yang
- Fei Huang
- Jiaqi Chen
- Yuanmei Guo (ORCID: https://orcid.org/0000-0003-0381-9647)
- Xiaoxiao Zou
- Junwu Ma
- Yang Wen
- Tao Jiang
- Ying Su
- Biao Chen
- Xinkai Tong
- Zhimin Zhou
- Lin Rao
- Dong Chen
- Zhiyan Zhang
- Ruirong Liu
Institutions
- Jiangxi Agricultural University (CN)
Publication Details
- Journal
- Science
- Published
- 2026-03-26
- DOI
- https://doi.org/10.1126/science.adu7265
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 0.00