A Green and Ultrasensitive Spectrofluorimetric Approach for the Determination of Salbutamol: Investigation of Photo‐Induced Electron Transfer Process and Application to Spiked Human Plasma, Pharmaceutical Preparations, and Content Uniformity Assessme

The present study describes the development of a straightforward and sensitive spectrofluorimetric approach for analyzing salbutamol (SBT). It is a short-acting β₂-adrenergic agonist widely utilized in treating asthma and chronic obstructive pulmonary conditions. Several solvents, surfactants, and pH were studied, and their effects on the fluorescence activity of the drug were monitored. Fluorescence measurements were conducted using methanol as the diluting solvent, which significantly enhanced the emission signal recorded at 310 nm when excited at 280 nm. The method was validated following the International Council for Harmonization (ICH). The method demonstrated excellent linearity in concentrations ranging from 30 to 400 ng/mL, with a correlation coefficient of 0.9997. The detection limit was 6.7 ng/mL, and the quantitation limit was 20 ng/mL. The procedure exhibited high selectivity, showing no interfering liability from commonly used pharmaceutical excipients. Successful application of the method was achieved in the analysis of SBT in commercial dosage forms, spiked human plasma, and content uniformity testing. Moreover, the influence of pH on the intensity of fluorescence of SBT was used to determine the apparent pKa value of the drug. The environmental superiority of the present technique was confirmed through different green chemistry evaluation tools.