Molecular droplet digital <scp>PCR</scp> diagnostics and bioassays for monitoring insecticide resistance status in <scp> <i>Myzus persicae</i> </scp> populations from Greece

Myzus persicae in Greece exhibits a high level of genetic variability, leading to severe and dynamic resistance problems. The widespread metabolic resistance (CYP6CY3 overexpression) which has been found to compromise neonicotinoids and fluryradifurone and the emergence of flonicamid resistance complicate the implementation of integrated pest management. The ddPCR panel provides a valid, evidence-based tool for large-scale monitoring of resistance and decision-making, essential for managing this difficult to control pest. However, bioassays are also useful when molecular markers are not available. © 2026 Society of Chemical Industry.