Vaccination of mice with Trichinella spiralis serine proteinase enhanced gut epithelial barrier and elicited a high protective immunity

rTsSPc vaccination produced a high protective immunity through multiple synergistic mechanisms: eliciting an obvious humoral and cellular immunity, gut local mucosal sIgA responses; blocking the binding of parasite-derived TsSPc to gut RACK1 receptors and the activation of MAPK/ERK1/2 pathway, improved gut epithelial integrity, inhibiting larval invasion, enhancing macrophages' ability of ADCC killing larvae, and finally reduced parasite burden and alleviated inflammation of intestines and skeletal muscles. TsSPc might be a promising novel candidate target for anti-T. spiralis vaccine.