Vitamin A Alleviates the Reduction in Milk Fat Synthesis through the TrxR/PPARγ Pathway in H₂O₂-Injured BMECs <sup>.1</sup>
This study aimed to investigate the effect and mechanisms by which vitamin A (VA) alleviates the reduction in milk fat synthesis by silencing TrxR and blocking the PPAR in bovine mammary gland epithelium cells (BMECs) injured by H 2 O 2 .TrxR was silenced by using small interfering RNA (siRNA) to detect the role of TrxR in milk fat synthesis.The third-passage BMECs were allocated to 6 treatments including CON, H 2 O 2 , VA+H 2 O 2 , siTrxR, siTrxR + VA, and siTrxR + VA+H 2 O 2 , to evaluate the mechanism of VA alleviating the decline of milk fat synthesis by TrxR signaling pathway.Subsequently, GW9662 (GW) was used to inhibit PPAR activity.Cells were randomly assigned to 6 treatments including CON,
Authors
- Y. B. Chen
- Xi Zhang (ORCID: https://orcid.org/0000-0002-4638-3997)
- Xin Guo (ORCID: https://orcid.org/0000-0002-4470-5585)
- Ying Hao (ORCID: https://orcid.org/0000-0002-8008-9601)
- Jing Yan (ORCID: https://orcid.org/0000-0001-9154-1408)
- Yanli Zhao (ORCID: https://orcid.org/0000-0002-9231-8360)
- Sumei Yan
- Yongmei Guo
Institutions
- Inner Mongolia Agricultural University (CN)
- Inner Mongolia University (CN)
Publication Details
- Journal
- Animal Nutriomics
- Published
- 2026-01-01
- DOI
- https://doi.org/10.1017/anr.2026.10034
- Primary Topic
- Peroxisome Proliferator-Activated Receptors
- Type
- article
- Field-Weighted Citation Impact
- 0.00