Invitro Antioxidant, Antimicrobial and Bioassay Guided-Fractionation of Methanol Extract of Pericopsis Laxifora Leaf

This study investigated the in vitro antioxidant and antimicrobial activities of the Methanol extract of Pericopsis laxiflora leaf (MLPL) and its bioassay-guided fractions, aiming to identify the most active constituents. Phytochemical screening of MLPL and its fractions revealed the presence of flavonoids, anthraquinones, tannins, saponins, and alkaloids, with notable variations across fractions. Antioxidant activity was evaluated using DPPH radical scavenging, lipid peroxidation (MDA), nitric oxide, superoxide radical scavenging, and reducing power assays. The ethyl acetate (EAFPL) and butanol (BFPL) fractions consistently demonstrated superior antioxidant activity, with EAFPL showing the highest potency across all models, correlating with its high phenolic content. Antimicrobial activity was assessed against bacterial pathogens (Staphylococcus aureus, Streptococcus pneumoniae, Escherichia coli, Pseudomonas aeruginosa) and fungal strains (Candida albicans, Aspergillus spp.). EAFPL again exhibited the strongest inhibition, indicating broad-spectrum antimicrobial potential. The study highlights the relevance of bioassay-guided fractionation in isolating potent phytochemicals responsible for biological activity. Clinically, the antioxidant and antimicrobial properties of EAFPL suggest potential therapeutic applications in oxidative stress-related diseases and microbial infections. Overall, P. laxiflora leaves are a promising source of bioactive compounds which serve as intervention in the management of oxidative stress-linked diseases and microbial infections.