Genome sequence of protease-free <i>Bacillus</i> subtilis KO7-S strain created for protein production
<i>Bacillus</i> strains efficiently secrete heterologous recombinant proteins as well as multiple proteases, with the latter destroying the former. Strains deleted of multiple secreted proteases are, therefore, preferred as protein expression hosts. We report the genome sequence of one such easily available strain, <i>Bacillus subtilis</i> KO7-S.
Authors
- Daniel R. Zeigler (ORCID: https://orcid.org/0000-0003-2332-3294)
- Ankur Bothra (ORCID: https://orcid.org/0000-0002-9038-740X)
- Skyler A. Kuhn (ORCID: https://orcid.org/0000-0003-0606-2125)
- Ryan Routsong (ORCID: https://orcid.org/0000-0002-1348-6172)
- Stephen H. Leppla
Institutions
- National Institutes of Health (US)
- National Institute of Allergy and Infectious Diseases (US)
- The Ohio State University (US)
Publication Details
- Journal
- Microbiology Resource Announcements
- Published
- 2026-03-23
- DOI
- https://doi.org/10.1128/mra.00016-26
- Primary Topic
- Bacterial Genetics and Biotechnology
- Type
- article
- Field-Weighted Citation Impact
- 0.00