Effect of Limonene and a Cinnamaldehyde–Carvacrol Blend on the Fermentation, Nutritional Quality, and Aerobic Stability of Corn Silage

This study evaluated the effects of essential oils, such as limonene (LIM) and a cinnamaldehyde–carvacrol blend (CCB), on the fermentation, chemical composition, in vitro digestibility, and aerobic stability of corn silage stored for 150 and 200 days. Treatments included a control, CCB (100 and 200 mg/kg DM), and LIM (100 and 200 mg/kg DM). Essential oils improved fermentation by increasing lactic acid (up to 7.46% of DM) and reducing proteolysis (NH3-N: 0.46–0.59% of total N). Limonene, particularly at 100 mg/kg DM, enhanced the lactic:acetic acid ratio (up to 3.07), better preserved non-fiber carbohydrates (≈32.7%), and increased in vitro DM digestibility (up to 81.5%) compared to the control (≈76.0%). The CCB treatment raised acetic acid concentrations (up to 3.04% of DM). Extending storage to 200 days reduced DM recovery (≈84.0%) versus 150 days (92.5%). Treated silages showed greater aerobic stability, with a lower pH increase after 72 h of air exposure, most notably in the LIM 200 treatment (pH 4.10) compared to the control (pH 5.40). Essential oils, particularly limonene, effectively improve the fermentative quality, nutritional value, and aerobic stability of corn silage under prolonged storage.

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Publication Details

Journal
Fermentation
Published
2026-03-21
DOI
https://doi.org/10.3390/fermentation12030167
Primary Topic
Ruminant Nutrition and Digestive Physiology
Type
article
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article

Effect of Limonene and a Cinnamaldehyde–Carvacrol Blend on the Fermentation, Nutritional Quality, and Aerobic Stability of Corn Silage

Giuliano Reis Pereira Muglia, Ronnie Coêlho de Andrade, Mábio Silvan José da Silva, Isabele Paola de Oliveira Amaral et al.
Fermentation
Ruminant Nutrition and Digestive Physiology
article

Effect of Limonene and a Cinnamaldehyde–Carvacrol Blend on the Fermentation, Nutritional Quality, and Aerobic Stability of Corn Silage

Giuliano Reis Pereira Muglia, Ronnie Coêlho de Andrade, Mábio Silvan José da Silva, Isabele Paola de Oliveira Amaral, Marco Antonio Previdelli Orrico Junior, Ana Carolina Amorim Orrico
article en

Abstract

This study evaluated the effects of essential oils, such as limonene (LIM) and a cinnamaldehyde–carvacrol blend (CCB), on the fermentation, chemical composition, in vitro digestibility, and aerobic stability of corn silage stored for 150 and 200 days. Treatments included a control, CCB (100 and 200 mg/kg DM), and LIM (100 and 200 mg/kg DM). Essential oils improved fermentation by increasing lactic acid (up to 7.46% of DM) and reducing proteolysis (NH3-N: 0.46–0.59% of total N). Limonene, particularly at 100 mg/kg DM, enhanced the lactic:acetic acid ratio (up to 3.07), better preserved non-fiber carbohydrates (≈32.7%), and increased in vitro DM digestibility (up to 81.5%) compared to the control (≈76.0%). The CCB treatment raised acetic acid concentrations (up to 3.04% of DM). Extending storage to 200 days reduced DM recovery (≈84.0%) versus 150 days (92.5%). Treated silages showed greater aerobic stability, with a lower pH increase after 72 h of air exposure, most notably in the LIM 200 treatment (pH 4.10) compared to the control (pH 5.40). Essential oils, particularly limonene, effectively improve the fermentative quality, nutritional value, and aerobic stability of corn silage under prolonged storage.

FermentationVol. 12(3)
Universidade Federal da Grande Dourados (BR)
Zero hunger
Openalex Percentile: Top 10%
Ruminant Nutrition and Digestive Physiology
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