Vibrio glycogenivorans sp. nov., a marine bacterium isolated from the surface seawater of the South China Sea

Two Gram-stain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacteria, designated strains WXL103 and WXL210^T, were isolated from surface seawater which was collected from the South China Sea. Strain WXL103 was most closely related to <i>Vibrio astriarenae</i> JCM 19233^T with 97.8% sequence similarity, and WXL210^T was also most closely related to <i>V. astriarenae</i> JCM 19233^T (97.7% sequence similarity). Phylogenetic analysis based on 16S rRNA genes, phylogenomic analysis of single-copy gene families and whole-genome data placed WXL103 and WXL210^T within the genus <i>Vibrio</i>, and they formed a close branch with <i>Vibrio agarivorans</i> CECT 5085^T and <i>V. astriarenae</i> JCM 19233^T. The growth of strains WXL103 and WXL210^T occurred at 10-42 °C (optimum 28 °C), at pH 7.0-9.0 (optimum 7.0-8.0) and in the presence of 2.5-8% (w/v) NaCl (optimum 3-4%). The predominant fatty acids (>10%) were C_{16 : 0}, summed feature 3 (C_{16 : 1} <i> ω</i>7c and/or C_{16 : 1} <i> ω</i>6c) and summed feature 8 (C_{18 : 1} <i> ω</i>7c and/or C_{18 : 1} <i> ω</i>6c). The DNA G+C contents of WXL103 and WXL210^T were 48.61 and 48.54 mol%, respectively. These two strains demonstrated average nucleotide identity values of 97.9% with each other, confirming that they were the same species. On the basis of the polyphasic evidence, strains WXL103 and WXL210^T are proposed to represent a novel species of the genus <i>Vibrio</i>, for which the name <i>Vibrio glycogenivorans</i> sp. nov. is proposed. The type strain is WXL210^T (KCTC 8711^T=MCCC 1K09445^T), and strain WXL103 is deposited in the culture collection centre with numbers KCTC 8767 and MCCC 1K09443.