Development and pilot application of a point-of-need molecular xenomonitoring protocol for tsetse (Glossina sp:) in a low-resource setting
This study demonstrated that sensitive molecular xenomonitoring of HAT and AAT pathogens can be carried out without the need for cold-chain storage or high-powered equipment. Further improvements to the system might be achieved by modifying the DNA extraction protocol to enable high-throughput or pooled samples, increasing the sensitivity of the T. b. gambiense DNA detection assay and exploring more sustainable power sources.
Authors
- Martha Betson (ORCID: https://orcid.org/0000-0002-4220-3290)
- Sophie Dunkley (ORCID: https://orcid.org/0000-0002-0638-7782)
- Iñaki Tirados (ORCID: https://orcid.org/0000-0002-9771-4880)
- Isabel Saldanha (ORCID: https://orcid.org/0000-0003-4141-8625)
- Gala Garrod (ORCID: https://orcid.org/0000-0002-7140-1077)
- Stephen J. Torr (ORCID: https://orcid.org/0000-0001-9550-4030)
- Andrew Hope (ORCID: https://orcid.org/0000-0001-8194-8917)
- Lucas J. Cunningham (ORCID: https://orcid.org/0000-0002-3017-4074)
- Edward Aziku
- Alex T. Trima
- Victor Drapari
- Henry Ombanya
- Albert Mugenyi
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2026-03-23
- DOI
- https://doi.org/10.1371/journal.pntd.0013706
- Primary Topic
- Trypanosoma species research and implications
- Type
- article
- Field-Weighted Citation Impact
- 0.00