Direct interaction between phosphotransferase LpxT and ArnT modulates polymyxin B resistance in <i>Pseudomonas aeruginosa</i>

Treatment of <i>Pseudomonas aeruginosa</i> infections is challenging due to its antibiotic resistance. Polymyxins are considered last-resort options for treating serious infections caused by multidrug-resistant <i>P. aeruginosa</i>. Understanding the molecular mechanisms of polymyxin resistance may provide clues for the development of new therapeutic strategies against <i>P. aeruginosa</i>. In this study, we demonstrated that the phosphatidic acid-phosphatase family (PAP2) protein LpxT interacts with L-Ara4N transferase ArnT and controls polymyxin B resistance in <i>P. aeruginosa</i>. Our findings reveal a novel role for lpxT and its molecular mechanism to defend against polymyxin B in <i>P. aeruginosa</i>.