Fluorescent bioreporters for assessing nitrogenase expression and rhizobial nodule occupancy in <i>Lotus japonicus</i> and <i>Pisum sativum</i>
A bioreporter system based on sfYFP, sfCFP, and mScarlet-I in <i>Mesorhizobium japonicum</i> and sfYFP and sfCFP in <i>Rhizobium leguminosarum</i>, driven by <i>nifH</i> consensus promoters was established to monitor occupancy and nitrogen fixation events within legume host nodules. These bioreporter plasmids expand established rhizobium sfGFP system to new hosts and fluorophores.
Authors
- Chandan Kumar Gautam (ORCID: https://orcid.org/0000-0001-5867-1868)
- Barney A. Geddes (ORCID: https://orcid.org/0000-0001-8309-2083)
- Gayathri Senanayake (ORCID: https://orcid.org/0000-0003-1597-6973)
- Amanda B. Pease (ORCID: https://orcid.org/0000-0001-9215-6499)
- Birgit M. Prüß
Institutions
- Dakota State University (US)
Publication Details
- Journal
- Microbiology Resource Announcements
- Published
- 2026-03-23
- DOI
- https://doi.org/10.1128/mra.00103-26
- Primary Topic
- Legume Nitrogen Fixing Symbiosis
- Type
- article
- Field-Weighted Citation Impact
- 0.00