Development and evaluation of an assay for the detection of tick-borne encephalitis virus RNA via real-time PCR with reverse transcription

The results indicate that the developed RT‒PCR assay is a reliable and competitive method for the detection of TBEV RNA, which establishes its value as a tool for diagnosing and monitoring the virus.

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Publication Details

Journal
Parasites & Vectors
Published
2026-03-23
DOI
https://doi.org/10.1186/s13071-026-07366-5
Primary Topic
Mosquito-borne diseases and control
Type
article
Field-Weighted Citation Impact
0.00
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article

Development and evaluation of an assay for the detection of tick-borne encephalitis virus RNA via real-time PCR with reverse transcription

Anna S. Gladkikh, Ivan S. Kholodilov, Alena А. Sharova, Anna S. Dolgova et al.
Parasites & Vectors
Mosquito-borne diseases and control
article

Development and evaluation of an assay for the detection of tick-borne encephalitis virus RNA via real-time PCR with reverse transcription

Anna S. Gladkikh, Ivan S. Kholodilov, Alena А. Sharova, Anna S. Dolgova, Galina G. Karganova, Anna V. Shabalina, Marina V. Safonova, Margarita Popova, Dmitry D. Naydenov, Svetlana Schirobokova, Vladimir Dedkov, Ekaterina Klyuchnikova, Tatiana Arbuzova, Edward Ramsay, Valeriya Sbarzaglia
article en

Abstract

The results indicate that the developed RT‒PCR assay is a reliable and competitive method for the detection of TBEV RNA, which establishes its value as a tool for diagnosing and monitoring the virus.

Parasites & Vectors
Sechenov University (RU), Saint Petersburg Pasteur Institute (RU), Federal Service for Surveillance on Consumer Rights Protection and Human Wellbeing (RU), Chumakov Institute of Poliomyelitis and Viral Encephalitides (RU)
Climate action
Openalex Percentile: Top 16%
Mosquito-borne diseases and control
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