Chromosome-level genome assemblies of Nicotiana attenuata (coyote tobacco) and Nicotiana obtusifolia (desert tobacco)

Nicotiana attenuata and Nicotiana obtusifolia are two wild tobacco species from the Solanaceae plant family, which produce diverse specialized metabolites and have been established as ecological model systems for studying plant-insect interactions. The previously published N. attenuata and N. obtusifolia genomes were draft assemblies with low assembly contiguity and a high proportion of gaps, limiting comparative genomic analysis. Here, we performed chromosome-level genome assemblies and annotations of these two species using long-read sequencing and Hi-C data. Both N. attenuata and N. obtusifolia genomes were anchored to 12 chromosomes, with assembled genome sizes of 2.2 Gb and 1.3 Gb, respectively. We achieved high BUSCO completeness at the protein level, with 99.3% for N. attenuata and 97.9% for N. obtusifolia. The reference genomes of N. attenuata and N. obtusifolia presented in this study will advance the understanding of metabolic innovations in Solanaceae plants.