A dual-signals peptide-based probe for fluorometric and colorimetric detection of copper(II) ions and glyphosate in multiple food samples and biological system

A new dual-signals peptide-based probe FAHK was rationally designed and successfully synthesized for sequential detection of Cu 2+ and glyphosate throughfluorescence and colorimetric sensing method. The limit of detections (LODs) for Cu 2+ and glyphosate were calculated to be 49.7 nM and 66.3 nM, respectively. Additionally, FAHK not only demonstrated excellent accuracy in the determination of real food, water and vegetables samples, but also was applied for fluorescence imaging Cu 2+ and glyphosate in two biological systems. Besides, test strips experiments showed that FAHK has extraordinary potential in environmental monitoring and food analysis applications. Meanwhile, we explored the application of FAHK in molecular logic gates, and a visual portable sensing platform was established via the smartphone color Picker App system, successfully achieving semi-quantitative analysis of Cu 2+ and glyphosate. More importantly, FAHK has successfully achieved the detection of glyphosate residues on six food surfaces and soil samples with satisfactory results. • Dual-signals peptide-based probe FAHK was fabricated for continuous detection of Cu 2+ and glyphosate. • The detection limit of 49.7 nM for Cu 2+ and 66.3 nM for glyphosate. • FAHK was applied for determination of Cu 2+ and glyphosate in biological system and food samples. • The practical utility FAHK was studied using test strips, logic gate and smartphone App . • FAHK achieved naked-eye detection of Cu 2+ and glyphosate residues on surfaces of food and soil.